DNA Mediated Energy Transfer from 4',6-Diamidino-2-phenylindole to Ru(II)[(1,10-phenanthroline)2L]2+: Effect of Ligand Structure 


Vol. 26,  No. 4, pp. 537-542, Apr.  2005
10.5012/bkcs.2005.26.4.537


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  Abstract

It was proposed that Ru(II)[(1,10-phenanthroline)2dipyrido[3,2-a:2',3'-c]phenazine ([Ru(phen)2DPPZ]2+) complexes and 4',6-diamidino-2-phenylindole (DAPI) simultaneously bind to poly[d(A-T)2] (Biophysics. J. 2003, 85, 3865). Forster type resonance energy transfer from excited DAPI to [Ru(phen)2DPPZ]2+ complexes was observed. In this study, we synthesized ?- and Λ-[Ru(phenanthroline)2dipyrido[3,2-a:2’,3’-c]6- azaphenazine] ([Ru(phen)2DPAPZ]2+) at which the DNA intercalating ligand DPPZ was replaced and we studied its binding properties to poly[d(A-T)2] in the presence and absence of DAPI using polarized spectroscopy and fluorescence techniques. All the spectroscopic properties of the [Ru(phen)2DPAPZ]2+- poly[d(A-T)2] complex were the same in the presence and absence of DAPI that blocks the minor groove of polynucleotide, suggesting both ?- and Λ-[Ru(phen)2DPAPZ]2+ complexes are located at the major groove of poly[d(A-T)2]. On the other hand, in contrast with [Ru(phen)2DPPZ]2+, both ?- and Λ-[Ru(phen)2DPAPZ]2+ exhibited almost twice the efficiency in the fluorescence quenching of DAPI that binds at the minor groove of poly[d(A-T)2]. This observation indicates that the efficiency of the Forster type resonance energy transfer can be controlled by a small change in the chemical structure of the intercalated ligand.

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  Cite this article

[IEEE Style]

M. R. Youn, S. J. Moon, B. W. Lee, D. Lee, J. Kim, S. K. Kim, C. Lee, "DNA Mediated Energy Transfer from 4',6-Diamidino-2-phenylindole to Ru(II)[(1,10-phenanthroline)2L]2+: Effect of Ligand Structure," Bulletin of the Korean Chemical Society, vol. 26, no. 4, pp. 537-542, 2005. DOI: 10.5012/bkcs.2005.26.4.537.

[ACM Style]

Mi Ryung Youn, Seok Joon Moon, Bae Wook Lee, Dong-Jin Lee, Jong-Moon Kim, Seog K. Kim, and Chong-Soon Lee. 2005. DNA Mediated Energy Transfer from 4',6-Diamidino-2-phenylindole to Ru(II)[(1,10-phenanthroline)2L]2+: Effect of Ligand Structure. Bulletin of the Korean Chemical Society, 26, 4, (2005), 537-542. DOI: 10.5012/bkcs.2005.26.4.537.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949