Microfluidic Immunoassay Platform Using Antibody-immobilized Glass Beads and Its Application for Detection of Escherichia coli O157:H7 


Vol. 27,  No. 4, pp. 479-483, Apr.  2006
10.5012/bkcs.2006.27.4.479


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  Abstract

We developed a microfluidic immunoassay platform for the detection of various analytes such as bacterial pathogen by packing antibody-immobilized glass beads in spatially-isolated microchambers on a microfluidic device. Primary amines of antibody were covalently conjugated to carboxyl-terminated glass beads previously treated with aminosilane followed by glutaraldehyde. Through this covalent binding, up to 905 μg immunoglobulin G (IgG) per gram of glass beads was immobilized. For application, glass beads attaching antibody specific to Escherichia coli O157:H7, a foodborne pathogen, were packed into a microfluidic device and used for the detection of the serotype. This prototype immunoassay device can be used for the simultaneous detection of multiple analytes by sequentially packing different-sized glass beads attaching different antibody in discrete microchambers on a single microfluidic device.

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  Cite this article

[IEEE Style]

N. Y. Lee, Y. Yang, Y. S. Kim, S. Park, "Microfluidic Immunoassay Platform Using Antibody-immobilized Glass Beads and Its Application for Detection of Escherichia coli O157:H7," Bulletin of the Korean Chemical Society, vol. 27, no. 4, pp. 479-483, 2006. DOI: 10.5012/bkcs.2006.27.4.479.

[ACM Style]

Nae Yoon Lee, Yoonsun Yang, Youn Sang Kim, and Sungsu Park. 2006. Microfluidic Immunoassay Platform Using Antibody-immobilized Glass Beads and Its Application for Detection of Escherichia coli O157:H7. Bulletin of the Korean Chemical Society, 27, 4, (2006), 479-483. DOI: 10.5012/bkcs.2006.27.4.479.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949