Label-Free and Real-Time Monitoring of Phosphatase Reactions Using a Phosphate-Specific and Fluorescent Probe 


Vol. 29,  No. 5, pp. 943-947, May  2008
10.5012/bkcs.2008.29.5.943


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  Abstract

A phosphate-specific and fluorescent probe was prepared for label-free phosphatase assays based on fluorescence polarization. By using the probe, dephosphorylation reactions of DNA and protein substrates by calf intestinal alkaline phosphatase (CIP) could effectively be monitored in real-time. Since this assay method does not require additional materials such as labeled substrates and phosphospecific antibodies to obtain fluorescence polarization signals, it is simple, cost-effective, and expected to be useful not only for measuring activity of phosphatases but also for high-throughput screening of phosphatase inhibitors.

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  Cite this article

[IEEE Style]

J. Lee, H. Ahn, D. Shin, D. Ahn, "Label-Free and Real-Time Monitoring of Phosphatase Reactions Using a Phosphate-Specific and Fluorescent Probe," Bulletin of the Korean Chemical Society, vol. 29, no. 5, pp. 943-947, 2008. DOI: 10.5012/bkcs.2008.29.5.943.

[ACM Style]

Jihoon Lee, Hee-Chul Ahn, Dongyun Shin, and Dae-Ro Ahn. 2008. Label-Free and Real-Time Monitoring of Phosphatase Reactions Using a Phosphate-Specific and Fluorescent Probe. Bulletin of the Korean Chemical Society, 29, 5, (2008), 943-947. DOI: 10.5012/bkcs.2008.29.5.943.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949