Biological Characterization of the Omp1-like Protein from Actinobacillus actinomycetemcomitans 


Vol. 31,  No. 2, pp. 275-280, Feb.  2010
10.5012/bkcs.2010.31.2.275


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  Abstract

Actinobacillus actinomycetemcomitans is a gram-negative, nonmotile coccobacillus bacterium that is associated with several human diseases, including endocarditis, meningitis, osteomyelitis, subcutaneous abscesses and periodontal diseases. A full-length Omp1-like protein gene from A. actinomycetemcomitans was cloned into a pQE30 vector and overexpressed in Escherichia coli BL21(DE3) cells. The protein revealed sequence homologies to Seventeen kilodalton proteins (Skp) from Pasteurella multocida and E. coli that have been characterized as periplasmic chaperones. This soluble Omp1-like protein was successfully purified to homogeneity for further folding and functional studies. The purity, identity, and conformation of the protein were determined using sodium dodecyl sulfate polyacrylamide gel electrophoresis, matrix-assisted laser desorption ionization mass spectrometry, circular dichroism, fluorescence spectroscopic, and differential scanning calorimetric studies. We showed that the protein formed an oligomer larger than a tetramer. We found, further, that it is comprised of mostly α-helices and boasts high thermal stability.

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  Cite this article

[IEEE Style]

J. H. Ha, M. S. Jeong, W. S. Jo, M. H. Jeong, S. B. Jang, "Biological Characterization of the Omp1-like Protein from Actinobacillus actinomycetemcomitans," Bulletin of the Korean Chemical Society, vol. 31, no. 2, pp. 275-280, 2010. DOI: 10.5012/bkcs.2010.31.2.275.

[ACM Style]

Jung Hye Ha, Mi Suk Jeong, Wol Soon Jo, Min Ho Jeong, and Se Bok Jang. 2010. Biological Characterization of the Omp1-like Protein from Actinobacillus actinomycetemcomitans. Bulletin of the Korean Chemical Society, 31, 2, (2010), 275-280. DOI: 10.5012/bkcs.2010.31.2.275.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949