A Linear Beacon System Featuring an Internal Deoxyguanine Quencher Allows Highly Selective Detection of Single Base Mismatches 


Vol. 31,  No. 7, pp. 2011-2014, Jul.  2010
10.5012/bkcs.2010.31.7.2011


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  Abstract

The fluorescence intensity of a single-stranded oligonucleotide containing a fluorene-labeled deoxyuridine (UFl) unit increases by only 1.5-fold upon formation of its perfectly matched duplex. To increase the fluorescence signal during hybridization, we positioned a quencher strand containing a deoxyguanine (dG) nucleobase, functioning as an internal quencher, opposite to the UFl unit to reduce the intrinsic fluorescence upon hybridization with a probe. From an investigation of the optimal length of the quencher strand and the effect of the neighboring base sequence, we found that a short strand (five-nucleotide) containing all natural nucleotides and dG as an internal quencher was effective at reducing the intrinsic fluorescence of a linear beacon; it also exhibited high total discrimination factors for the formation of perfectly matched and single base-mismatched duplexes. Such assays that function based on clear changes in fluorescence in response to single-base nucleotide mutations would be useful tools for accelerating diagnoses related to various diseases.

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  Cite this article

[IEEE Style]

Y. A. Lee and G. T. Hwang, "A Linear Beacon System Featuring an Internal Deoxyguanine Quencher Allows Highly Selective Detection of Single Base Mismatches," Bulletin of the Korean Chemical Society, vol. 31, no. 7, pp. 2011-2014, 2010. DOI: 10.5012/bkcs.2010.31.7.2011.

[ACM Style]

Young Ae Lee and Gil Tae Hwang. 2010. A Linear Beacon System Featuring an Internal Deoxyguanine Quencher Allows Highly Selective Detection of Single Base Mismatches. Bulletin of the Korean Chemical Society, 31, 7, (2010), 2011-2014. DOI: 10.5012/bkcs.2010.31.7.2011.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949