Biochemical and Biophysical Characterizations of the Interaction between Two PDZ Adapter Proteins NHERF and E3KARP in vitro 


Vol. 31,  No. 11, pp. 3241-3246, Nov.  2010
10.5012/bkcs.2010.31.11.3241


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  Abstract

NHERF (Na+/H+ exchanger regulatory factor) and E3KARP (NHE3 kinase A regulatory protein) play important roles in membrane targeting, trafficking and sorting of ion channels, transmembrane receptors and signaling proteins in many tissues. Each of these proteins contains two PDZ (PSD-95/Dlg-1/ZO-1) domains, which mediate the assembly of transmembrane and cytosolic proteins into functional signal transduction complexes. The interaction between NHERF and E3KARP was investigated by surface plasmon resonance spectroscopy (BIAcore), fluorescence measurement, His-tagged pull-down experiment, and size-exclusion column (SEC) chromatography. BIAcore experiments revealed that NHERF bound to E3KARP with an apparent K

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  Cite this article

[IEEE Style]

E. Y. Hwang, M. S. Jeong, S. B. Jang, "Biochemical and Biophysical Characterizations of the Interaction between Two PDZ Adapter Proteins NHERF and E3KARP in vitro," Bulletin of the Korean Chemical Society, vol. 31, no. 11, pp. 3241-3246, 2010. DOI: 10.5012/bkcs.2010.31.11.3241.

[ACM Style]

Eun Young Hwang, Mi Suk Jeong, and Se Bok Jang. 2010. Biochemical and Biophysical Characterizations of the Interaction between Two PDZ Adapter Proteins NHERF and E3KARP in vitro. Bulletin of the Korean Chemical Society, 31, 11, (2010), 3241-3246. DOI: 10.5012/bkcs.2010.31.11.3241.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949