Imaging the Enzymatic Reaction of Urease Using Liquid Crystal-Based pH Sensor 


Vol. 32,  No. 12, pp. 4377-4381, Dec.  2011
10.5012/bkcs.2011.32.12.4377


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  Abstract

In this study, real-time and label-free methods for monitoring the enzymatic reaction of urease, which releases ammonia through the hydrolysis of urea in an aqueous solution, were developed using a liquid crystal (LC)- based pH sensor. Nematic liquid crystal 4-cyano-4'-pentylbiphenyl (5CB), doped with 4'-pentyl-biphenyl-4- carboxylic acid (PBA), exhibited a shift in optical appearance from bright to dark when it was in contact with ammonia generated from the enzymatic reaction between urease and urea. This optical change was attributed to the anchoring transitions of LCs caused by hydrophobic interactions between the tails of deprotonted PBA (PBA−) molecules and the LCs at the aqueous/LC interface. This novel technique holds great promise for the sensitive detection of urease along with its substrates and inhibitors.

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  Cite this article

[IEEE Style]

Q. Hu and C. Jang, "Imaging the Enzymatic Reaction of Urease Using Liquid Crystal-Based pH Sensor," Bulletin of the Korean Chemical Society, vol. 32, no. 12, pp. 4377-4381, 2011. DOI: 10.5012/bkcs.2011.32.12.4377.

[ACM Style]

Qiong-Zheng Hu and Chang-Hyun Jang. 2011. Imaging the Enzymatic Reaction of Urease Using Liquid Crystal-Based pH Sensor. Bulletin of the Korean Chemical Society, 32, 12, (2011), 4377-4381. DOI: 10.5012/bkcs.2011.32.12.4377.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949