The Effect of the Oxygen Scavenging System on the pH of Buffered Sample Solutions: in the Context of Single-molecule Fluorescence Measurements 


Vol. 33,  No. 3, pp. 958-962, Mar.  2012
10.5012/bkcs.2012.33.3.958


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  Abstract

In single-molecule fluorescence experiment, the oxygen scavenging system is indispensable for avoiding photo-bleaching of fluorescent dyes. Here we report that the gloxy-based oxygen scavenging system commonly used in single molecule fluorescence experiments can disturb the solution pH considerably. To track in situ pH change, we utilized the pH-sensitive conformational transition of i-motif and examined the transition with ensemble and single-molecule FRET measurements. Based on our results, we also suggested several practical remedies for the stability of the solution pH.

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  Cite this article

[IEEE Style]

S. E. Kim, I. B. Lee, S. Hong, "The Effect of the Oxygen Scavenging System on the pH of Buffered Sample Solutions: in the Context of Single-molecule Fluorescence Measurements," Bulletin of the Korean Chemical Society, vol. 33, no. 3, pp. 958-962, 2012. DOI: 10.5012/bkcs.2012.33.3.958.

[ACM Style]

Sung Eun Kim, Il Buem Lee, and Seok-Cheol Hong. 2012. The Effect of the Oxygen Scavenging System on the pH of Buffered Sample Solutions: in the Context of Single-molecule Fluorescence Measurements. Bulletin of the Korean Chemical Society, 33, 3, (2012), 958-962. DOI: 10.5012/bkcs.2012.33.3.958.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949