Low-ε Static Probe Development for 15N-1H Solid-state NMR Study of Membrane Proteins for an 800 MHz NB Magnet 


Vol. 34,  No. 3, pp. 823-826, Mar.  2013
10.5012/bkcs.2013.34.3.823


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  Abstract

A low-ε solid-state NMR(Nuclear Magnetic Resonance) probe was developed for the spectroscopic analysis of two-dimensional 15N-1H heteronuclear dipolar coupling in dilute membrane proteins oriented in hydrated and dielectrically lossy lipid environments. The system employed a 800 MHz narrow-bore magnet. A solenoid coil strip shield was used to reduce deleterious RF sample heating by minimizing the conservative electric fields generated by the double-tuned resonator at high magnetic fields. The probe's design, construction, and performance in solid-state NMR experiments at high magnetic fields are described here. Such high-resolution solid-state NMR spectroscopic analysis of static oriented samples in hydrated phospholipid bilayers or bicelles could aid the structural analysis of dilute biological membrane proteins.

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  Cite this article

[IEEE Style]

T. Park, S. Choi, J. Jung, Y. Park, Y. Kim, "Low-ε Static Probe Development for 15N-1H Solid-state NMR Study of Membrane Proteins for an 800 MHz NB Magnet," Bulletin of the Korean Chemical Society, vol. 34, no. 3, pp. 823-826, 2013. DOI: 10.5012/bkcs.2013.34.3.823.

[ACM Style]

Tae-Joon Park, Sung-Sub Choi, Ji-Ho Jung, Yu-Geun Park, and Yongae Kim. 2013. Low-ε Static Probe Development for 15N-1H Solid-state NMR Study of Membrane Proteins for an 800 MHz NB Magnet. Bulletin of the Korean Chemical Society, 34, 3, (2013), 823-826. DOI: 10.5012/bkcs.2013.34.3.823.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949