Histidine (His83) is Essential for Heat Shock Factor 1 (HSF1) Activation in Protecting against Acid pH Stress 


Vol. 34,  No. 11, pp. 3405-3409, Nov.  2013
10.5012/bkcs.2013.34.11.3405


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  Abstract

The activation of heat shock factor 1 (HSF1) can be induced by the changes in environmental pH, but the mechanism of HSF1 activation by acidification is not completely understood. This paper reports that a low pH (pH~6.0) can trigger human HSF1 activation. Considering the involvement of the imidazole group of histidine residues under acid pH stress, an in vitro EMSA experiment, Trp-fluorescence spectroscopy, and protein structural analysis showed that the residue, His83, is the essential for pH-dependent human HSF1-activation. To determine the roles of His83 in the HSF1-mediated stress response affecting the cellular acid resistance, mouse embryo fibroblasts with normal wild-type or mutant mouse HSF1 expression were preconditioned by heating or pH stress. The results suggest that His83 is essential for HSF1 activation or the HSF1-mediated transcription of heat shock proteins, in protecting cells from acid pH stress.

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  Cite this article

[IEEE Style]

M. Lu, Z. Chang, J. Park, "Histidine (His83) is Essential for Heat Shock Factor 1 (HSF1) Activation in Protecting against Acid pH Stress," Bulletin of the Korean Chemical Society, vol. 34, no. 11, pp. 3405-3409, 2013. DOI: 10.5012/bkcs.2013.34.11.3405.

[ACM Style]

Ming Lu, Ziwei Chang, and Jang-Su Park. 2013. Histidine (His83) is Essential for Heat Shock Factor 1 (HSF1) Activation in Protecting against Acid pH Stress. Bulletin of the Korean Chemical Society, 34, 11, (2013), 3405-3409. DOI: 10.5012/bkcs.2013.34.11.3405.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949