Protective Effect Against Hydroxyl Radical-induced DNA Damage and Antioxidant Mechanism of [6]-gingerol: A Chemical Study 


Vol. 35,  No. 6, pp. 1633-1638, Jun.  2014
10.5012/bkcs.2014.35.6.1633


PDF
  Tumbnail

  Abstract

[6]-Gingerol is known as the major bioactive constituent of ginger. In the study, it was observed to effectively protect against •OH-induced DNA damage (IC50 328.60 ± 24.41 μM). Antioxidant assays indicated that [6]- gingerol could efficiently scavenge various free radicals, including •OH radical (IC50 70.39 ± 1.23 μM), •O2 − radical (IC50 228.40 ± 9.20 μM), DPPH• radical (IC50 27.35 ± 1.44 μM), and ABTS+• radical (IC50 2.53 ± 0.070 μM), and reduce Cu2+ ion (IC50 11.97 ± 0.68 μM). In order to investigate the possible mechanism, the reaction product of [6]-gingerol and DPPH• radical was further measured using HPLC combined mass spectrometry. The product showed a molecular ion peak at m/z 316 [M+Na]+, and diagnostic fragment loss (m/z 28) for quinone. On this basis, it can be concluded that: (i) [6]-gingerol can effectively protect against •OH-induced DNA damage; (ii) a possible mechanism for [6]-gingerol to protect against oxidative damage is •OH radical scavenging; (iii) [6]-gingerol scavenges •OH radical through hydrogen atom (H•) transfer (HAT) and sequential electron (e) proton transfer (SEPT) mechanisms; and (iv) both mechanisms make [6]-gingerol be oxidized to semi-quinone or quinone forms.

  Statistics
Cumulative Counts from November, 2022
Multiple requests among the same browser session are counted as one view. If you mouse over a chart, the values of data points will be shown.


  Cite this article

[IEEE Style]

J. Lin, X. Li, a, L. Chen, W. Lu, X. Chen, L. Han, D. Chen, "Protective Effect Against Hydroxyl Radical-induced DNA Damage and Antioxidant Mechanism of [6]-gingerol: A Chemical Study," Bulletin of the Korean Chemical Society, vol. 35, no. 6, pp. 1633-1638, 2014. DOI: 10.5012/bkcs.2014.35.6.1633.

[ACM Style]

Jing Lin, Xican Li, a, Li Chen, Weizhao Lu, Xianwen Chen, Lu Han, and Dongfeng Chen. 2014. Protective Effect Against Hydroxyl Radical-induced DNA Damage and Antioxidant Mechanism of [6]-gingerol: A Chemical Study. Bulletin of the Korean Chemical Society, 35, 6, (2014), 1633-1638. DOI: 10.5012/bkcs.2014.35.6.1633.

Search
(IN TITLE, AUTHOR, ABSTRACT,KEYWORDS)

Advanced Search ▼

POPULAR KEYWORDS
(TOP 10 KEYWORDS)

Recent Publications
(LAST 3 YEARS)

ISSN(Print) 0253-2964
ISSN(Online) 1229-5949