Synchrotron-based Transmission X-ray Microscopy (TXM) Observations of Fully Hydrated Blood Platelets and Their Activation Process 


Vol. 35,  No. 9, pp. 2625-2629, Sep.  2014
10.5012/bkcs.2014.35.9.2625


PDF
  Tumbnail

  Abstract

Platelets are anuclear discoid-shaped blood cells with key roles in human body. To understand the mechanisms of their activation process, it is required to have analytical imaging techniques capable of acquiring platelet images under fully hydrated conditions. Herein, for the first time, we demonstrate the capability of synchrotronbased transmission X-ray microscopy (TXM) to study platelets (resting and ADP activated) under hydrated and air-dried conditions. To confirm the biological imaging capability of TXM, fixed platelets were imaged and compared with whole mount electron microscopy (EM) images. TXM provided morphological information with sufficient spatial resolution with simple and quick sample preparation procedure. We also observed temporal changes during the platelet activation, which initially had a discoid shape (0 s), formed pseudopodia (30 s) and generated a network of fibrin (5 min). Our results clearly demonstrate the potential of TXM technique to study fully hydrated biological samples under in situ conditions.

  Statistics
Cumulative Counts from November, 2022
Multiple requests among the same browser session are counted as one view. If you mouse over a chart, the values of data points will be shown.


  Cite this article

[IEEE Style]

N. Yang, H. W. Nho, Y. Kalegowda, J. B. Kim, J. Song, H. Shin, T. H. Yoon, "Synchrotron-based Transmission X-ray Microscopy (TXM) Observations of Fully Hydrated Blood Platelets and Their Activation Process," Bulletin of the Korean Chemical Society, vol. 35, no. 9, pp. 2625-2629, 2014. DOI: 10.5012/bkcs.2014.35.9.2625.

[ACM Style]

Nuri Yang, Hyun Woo Nho, Yogesh Kalegowda, Jin Bae Kim, Jaewoo Song, Hyun-Joon Shin, and Tae Hyun Yoon. 2014. Synchrotron-based Transmission X-ray Microscopy (TXM) Observations of Fully Hydrated Blood Platelets and Their Activation Process. Bulletin of the Korean Chemical Society, 35, 9, (2014), 2625-2629. DOI: 10.5012/bkcs.2014.35.9.2625.

Search
(IN TITLE, AUTHOR, ABSTRACT,KEYWORDS)

Advanced Search ▼

POPULAR KEYWORDS
(TOP 10 KEYWORDS)

Recent Publications
(LAST 3 YEARS)

ISSN(Print) 0253-2964
ISSN(Online) 1229-5949