Liquid Crystal Droplet Patterns to Monitor Catalase Activity at Femtomolar Levels 


Vol. 35,  No. 9, pp. 2704-2710, Sep.  2014
10.5012/bkcs.2014.35.9.2704


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  Abstract

Catalase (CAT) decomposes hydrogen peroxide that is toxic to the body. In this study, simple and sensitive detector has been developed for observing catalase activity using liquid crystal droplet system. Microscale LC droplet patterns are formed by spreading aldehyde-doped nematic liquid crystal on pre-treated glass slides. When hydrogen peroxide is added, aldehyde is oxidized and amphiphiles are formed. Dodecanoates cause the pattern to transit from bright to dark as they self-assemble to form a carboxyalte monolayer at the interface. When a drop of pre-incubated CAT and hydrogen peroxide mixture is placed onto the pattern, bright fan-shape is observed. This planar optical appearance indicates that catalase has decomposed hydrogen peroxide. Compared to the detectors that have been previously developed, this system is more sensitive with detection limit of 1fM. This research suggests further studies to be on LC droplet patterning to develop highly sensitive and methodologically simple sensors for various chemicals.

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  Cite this article

[IEEE Style]

S. Yoon and C. Jang, "Liquid Crystal Droplet Patterns to Monitor Catalase Activity at Femtomolar Levels," Bulletin of the Korean Chemical Society, vol. 35, no. 9, pp. 2704-2710, 2014. DOI: 10.5012/bkcs.2014.35.9.2704.

[ACM Style]

Stephanie Yoon and Chang-Hyun Jang. 2014. Liquid Crystal Droplet Patterns to Monitor Catalase Activity at Femtomolar Levels. Bulletin of the Korean Chemical Society, 35, 9, (2014), 2704-2710. DOI: 10.5012/bkcs.2014.35.9.2704.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949