Graphene Oxide-based Direct Measurement of DNase I Activity with Single Stranded DNA 


Vol. 35,  No. 9, pp. 2749-2752, Sep.  2014
10.5012/bkcs.2014.35.9.2749


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  Abstract

Recent studies have shown that single-stranded DNA adsorbed onto graphene oxide is protected from DNase I cleavage. However, double-stranded DNA bound to graphene oxide and could be digested by DNase I. To elucidate whether single-stranded DNA is protect from DNase I in the presence of graphene oxide, this study conducted DNase I digestion using single-stranded DNA and single-stranded DNA containing the duplex region in the presence of graphene oxide. Addition of DNase I resulted in restoration of the fluorescence emission that had been quenched when DNA was adsorbed to graphene oxide. It indicates that DNase I cleaved the adsorbed single-stranded DNA onto graphene oxide, which was sufficient for the detection of DNase I activity.

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  Cite this article

[IEEE Style]

J. Gang, "Graphene Oxide-based Direct Measurement of DNase I Activity with Single Stranded DNA," Bulletin of the Korean Chemical Society, vol. 35, no. 9, pp. 2749-2752, 2014. DOI: 10.5012/bkcs.2014.35.9.2749.

[ACM Style]

Jongback Gang. 2014. Graphene Oxide-based Direct Measurement of DNase I Activity with Single Stranded DNA. Bulletin of the Korean Chemical Society, 35, 9, (2014), 2749-2752. DOI: 10.5012/bkcs.2014.35.9.2749.

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ISSN(Print) 0253-2964
ISSN(Online) 1229-5949