Enhancement of Antibacterial Activity of Short Tryptophan-rich Antimicrobial Peptide Pac-525 by Replacing Trp with His(chx) 


Vol. 35,  No. 9, pp. 2818-2824, Sep.  2014
10.5012/bkcs.2014.35.9.2818


PDF
  Tumbnail

  Abstract

Trp residue is considered as one of the important constituents in antimicrobial peptides (AMPs) as it presence secured good activities in many cases. However, it is preferable to be changed because of their sensitivity towards light. We have synthesized the short Trp-rich AMP Pac-525 and its analogues to investigate the possibility of His(chx) as possible replacement analogue for Trp in AMPs. Based on the assay result of the antibacterial activity including anti-MRSA activity, His(chx) is considered as good candidate for the Trp replacement. Through these study, we found that His(chx) had several merits to design therapeutic antimicrobial agents compare to Trp in terms of i) increasing antibacterial activity without hemolytic activity, ii) successful in designing the short peptide (only four residues), iii) having anti-MRSA activity, iv) overcoming the light sensitivity. Furthermore, transmission electron microscopy (TEM) and dye leakage experiments suggested that P11 and P16 containing His(chx) kill bacteria via forming pore/ion channels on bacterial cell membranes.

  Statistics
Cumulative Counts from November, 2022
Multiple requests among the same browser session are counted as one view. If you mouse over a chart, the values of data points will be shown.


  Cite this article

[IEEE Style]

M. Ahn, G. Rajasekaran, G. P, E. k. Ryu, G. Lee, J. Hyun, C. Cheong, N. Kim, S. Y. Shin, J. Bang, "Enhancement of Antibacterial Activity of Short Tryptophan-rich Antimicrobial Peptide Pac-525 by Replacing Trp with His(chx)," Bulletin of the Korean Chemical Society, vol. 35, no. 9, pp. 2818-2824, 2014. DOI: 10.5012/bkcs.2014.35.9.2818.

[ACM Style]

Mija Ahn, Ganesan Rajasekaran, Gunasekaran P, Eun kyoung Ryu, Ga-hyang Lee, Jae-Kyung Hyun, Chaejoon Cheong, Nam-Hyung Kim, Song Yub Shin, and Jeong-Kyu Bang. 2014. Enhancement of Antibacterial Activity of Short Tryptophan-rich Antimicrobial Peptide Pac-525 by Replacing Trp with His(chx). Bulletin of the Korean Chemical Society, 35, 9, (2014), 2818-2824. DOI: 10.5012/bkcs.2014.35.9.2818.

Search
(IN TITLE, AUTHOR, ABSTRACT,KEYWORDS)

Advanced Search ▼

POPULAR KEYWORDS
(TOP 10 KEYWORDS)

Recent Publications
(LAST 3 YEARS)

ISSN(Print) 0253-2964
ISSN(Online) 1229-5949